A Review on Aflatoxins Reduction in Food

Maryam Jalili


Aflatoxins (AFs) are cancerous secondary metabolites produced primarily by Aspergillus flavus and Aspergillus parasiticus in agricultural foodstuff such as peanuts, maize grains, cereals, and animal feeds. Food and Agricultural organization (FAO) estimated that as much as 25% of the world’s agricultural commodities are contaminated with mycotoxins, leading to significant economic losses. Moreover, AFs are highly toxic, mutagenic, teratogenic and carcinogenic. Therefore AFs reduction in food and feedstuffs is a major global concern. This review aims to bring up to date the detoxification methods applied for reduction of aflatoxins by physical (cleaning, heating, irradiation, adsorption), chemical (chemical compound, ozonization) and biological (applying bacteria, yeast and nontoxigenic Aspergillus strains) methods in different foods from 2000 to 2015. Papers related to aflatoxin reduction by managing aflatoxins risks, using resistant crops varieties, and good agricultural practices and papers related to other aflatoxins (M1, M2) were excluded.


Aflatoxin, Reduction, Physical method, Chemical method, Biological method

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Kabak B, Dobson ADW, Var I. Strategies to prevent mycotoxin contamination of food and animal feed: A review. Crit. Rev. Food Sci. Nutr. 2006; 46(8): 593-19.

Sforza S, Dall’Asta C, Marchelli R. Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry. Mass Spectrometry Rev. 2006; 25(1): 54-76.

Costanzo P, Santini A, Fattore L, Novellino E, Ritieni A. Toxicity of aflatoxin B1 towards the vitamin D receptor (VDR). Food Chem. Toxicol. 2015; 76(2): 77-9.

IARC. Monographs on the evaluation of carcinogenic risks to human. Some traditional herbal medicine, some mycotoxins, naphthalene and styrene. No. 82. IARC, Lyon, France

Wacoo AP, Wendiro D, Vuzi PC, Hawumba JF. Methods for Detection of Aflatoxins in Agricultural Food Crops. J. appl. chem. 2014; 2014: 11-15.

Villers F. Aflatoxins and safe storage, Front. Microbiol. 2014; 5: 158-64.

Ahmadzadeh F, Mirlohi M, Madani G. Reduction of aflatoxin M1 by Some Lactic Acid Bacteria and the Effect of pH and Temperature in Phosphate Buffer Saline Solution. J Chem. Bio. Phy. Sci. 2015; 3: 2748-2755.

Park JW, Kim YB. Effect of pressure cooking on aflatoxin B1 in rice. J. Agric. Food Chem. 2006; 54(6): 2431-35.

Velazhahan R, Vijayanandraj S, Vijayasamundeeswari A, Paranidharan V, Samiyappan R, Iwamoto T, Friebe B, Muthukrishnan S. Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill Structural analysis and biological toxicity of degradation product of aflatoxin G1. Food Cont. 2010; 21(5): 719–25.

Amezqueta S, Gonzalez-Penas E, Murillo M, Lopez de Cerain A. Occurrence of ochratoxin A in cocoa beans: Effect of shelling. Food Addit Contam. 2005; 22(6): 590–96.

Bucheli P, Taniwaki MH. Review Research on the origin, and on the impact of post-harvest handling and manufacturing on the presence of ochratoxin A in coffee. Food Addit Contam. 2002; 19(7): 655–65.

Fandohan P, Zoumenou D, Hounhouigan DJ, Marasas WF, Wingfield MJ, Hell K. Fate of aflatoxins and fumonisins during the processing of maize into food products in Benin. Int. J. Food Microbiol. 2005; 98(4): 249–59.

Turner P, Sylla A, Gong Y, Diallo M, Sutcliffe A, Hall A, Wild C. Reduction of exposure to carcinogenic aflatoxins by postharvest intervention measures in West Africa: a community-based study. Lancet, 2005; 365(9475):1950-59.

Jard, G; Liboz, T; Mathieu, F; Guyonvarch’h, A. and Lebrihi, A. Review of mycotoxin reduction in food and feed: from prevention in the field to detoxification by adsorption or transformation. Food Addit. Contamination Part A. Chem. Anal. Exposure Risk Assessment. 2011; 28(11): 1590-09.

Hwang JH, Lee KG. Reduction of aflatoxin B1 contamination in wheat by various cooking treatments. Food Chem. 2006; 98(1): 71–75.

Reddy UM, Rani PC. Effect of processing on detoxification of aflatoxins in maize. Indian J. Nutr. Diet. 2004; 43(1): 54-59.

Hussain A, Ali J, Akther S, Shafqatullah, Degradation of aflatoxins by roasting in contaminated peanuts. Pak. J. Biochem. Mol. Biol. 2011; 44(2): 56-59.

Mendez-Albores A, De Jesus-Flores F, Castaneda-Roldan E, Arambula-Villa G, Moreno- Martinez E. The effect of toasting and boiling on the fate of B-aflatoxins during pinole preparation. J. Food Engin. 2004; 65(4): 585.

Torres P, Guzman-Ortiz M, Ramirez-Wong B. Revising the role of pH and thermal treatments in aflatoxin content reduction during the tortilla and deep frying processes. J. Agric. Food Chem. 2001; 49(6): 2825-29.

De Obanos AP, Gonzalez-Penas E, De Cerain A. Influence of roasting and brew preparation on the ochratoxin A content in coffee infusion, Food Addit. Contam. 2005; 22(5): 463 – 71.

Raters MR, Matissek R. Thermal stability of aflatoxin B1 and ochratoxin A. Mycotoxin. Res. 2008; 24(3): 130-34.

Prado G, Carvalho EPD, Oliveira MS, Madeira JGC, Morais VD, Correa RF, Cardoso VN, Soares TV, Silva JFMD, Gonçalves RCP. Effect of gamma irradiation on the inactivation of aflatoxin B1 and fungal flora in peanut. Braz. J. Microbiol. 2003;34(suppl. 1):138-40.

Ahsan S, Hussain Z, Naqvi SA, Asi MR. Effect of gamma radiation on aflatoxin load, amino acid and fatty acid composition of Oryza Satival. Pak. J. Bot. 2013; 45(5): 1577-80.

Jalili M, Jinap S, Noranizan A. Effect of gamma radiation on reduction of mycotoxins in black pepper, Food Cont. 2010; 21(10): 1388–93

Ghanem I, Orfi M, Shamma M. Effect of gamma radiation on the inactivation of aflatoxin B1 in food and feed crops. Braz. J. Microbiol. 2008; 39(4): 787-91.

Jalili M, Jinap S, Noranizan A. Aflatoxins and ochratoxin a reduction in black and white pepper by gamma radiation. Rad. Physics. Chem.. 2012; 81(11): 1786–88

Aziz NH, Moussa LAA. Influence of gamma-radiation on mycotoxin producing moulds and mycotoxins in fruits. Food Cont. 2004; 13(4-5): 281–88.

Vita DS, Pitonzo Rosa P, Giuseppe A. Effect of Gamma Irradiation on Aflatoxins and Ochratoxin A Reduction in Almond Samples. J. Food Res. 2014; 3(4): 113-18.

Hasheminya SM, Dehghannya, J. Strategies for decreasing aflatoxin in livestock feed and milk. Intl. Res. J. Appl. Basic Sci.. 2013; 4(6): 1506-10.

Huwing A, Freimund S, Kappeli O, Dutler H. Mycotoxin detoxication of animal feed by different adsorbents. Toxicol. Lett. 2001; 122(2): 179–88.

Jaynes WF, Zartman RE. Aflatoxin toxicity reduction in feed by enhanced binding to surface-modified clay additives. Toxins (Basel). 2011; 3(5): 551-65.

Dakovic A, Tomasevic-Canovic M, Rottinghaus GE, Dondur V, Masic A. Adsorption of ochratoxin A on octadecyldimethyl benzyl ammonium exchanged-clinoptilolite-heulandite tuff. Colloids and Surfaces B: Biointerfaces. 2003; 30(1-2): 157–65.

Dakovic A, Tomasevic-Canovic M, Dondur V, Rottinghaus GE, Medakovic V, Zaric S. Adsorption of mycotoxins by organozeolites. Colloids and Surfaces B: Biointerface, 2005; 46(1): 20–25.

Jebali A, Hajesmail FH, Rodbari Mohamadi SH, Yadegari MH. Design of absorber column containing zeolite LTA for the removal of Aflatoxin. 2011. Conference: ECCMID 21st, ICC 27th

Khadem AA, Sharifi SD, Barati M, Borji M. Evaluation of the Effectiveness of Yeast, Zeolite and Active Charcoal as Aflatoxin Absorbents in Broiler Diets. Global Veterinaria. 2012; 8(4): 426-32.

Hekmati Moghaddam SH, Jebali A, Daliri K. The use og MgO-SiO2 nanocomposite for adsorption of aflatoxin in wheat flour samples. Nanoconference. 2014.

Jalili M, Jinap S, Radu S. The effect of chemical treatment on reduction of aflatoxins and ochratoxin A in black and white pepper during washing, Food Addit. Contam. 2011; 28(4): 485–93.

Pearson TC, Wicklow DT. Pasikatan MC. Reduction of aflatoxin and fumonisin contamination in yellow corn by high-speed dual-wavelenght sorting. Cereal Chem. 2004; 81(4): 490-98.

Whitaker TB, Dorner JW, Lamb M, Slate AB. The effect of sorting farmers’ stock peanuts by size and color on partitioning aflatoxin into various shelled peanut grade sizes. Peanut. Sci. 2005; 32(2):103–18.

Amezqueta S, Gonzalez-Penas E, Lizarraga T, Murillo-Arbizu M, Lopez DE, Cerain A. A Simple Chemical Method Reduces Ochratoxin A in Contaminated Cocoa Shells. J. Food Prot. 2008; 71(7):1422-26.

Tripathi S, Mishra HN. Studies on the efficacy of physical, chemical and biological aflatoxin B1 detoxification approaches in red chilli powder. Int. J. Food Safety. 2009; 2(1): 69 – 77.

Singh N, Jand SK, Baxi KK. Chemical detoxification of aflatoxins in contaminated poultry feed. Indian J Anim sci. 2003; 73(2):197-99.

Inan F, Pala M, Doymaz I. Use of ozone in detoxification of aflatoxin B1. in red pepper. J. Stored Product Res. 2007; 43(4): 425-29.

Aziz, N.H. & Youssef, B.M. Inactivation of naturally occurring of mycotoxins in some egyptian foods a and agricultural commodities by gamma-irradiation. Egypt. J .Food Sci. 2002;30(1): 167-77

Aquino S, Ferreira F, Ribeiro DHB, Corrêa B, Greiner R,Villavicencio ALCH. Evaluations of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize. Braz. J. Microbiol. 2005; 36(4): 352–56.

Mohamed NF, El-Dine RSS, Kotb MAM, Saber A. Assessing the Possible Effect of Gamma Irradiation on the Reduction of aflatoxin B1, and on the Moisture Content in Some Cereal Grains. Am. J. Biomed. Sci. 2015; 7(1): 33-39.

Mobeen AK, Aftab A, Asif A, Zuzzer AS. Aflatoxins B1 and B2 Contamination of Peanut and Peanut Products and Subsequent Microwave Detoxification. J. Pharm. Nutr. Sci. 2011; 1(1): 1-3.

Herzallah S, Alshawabkeh K, AL Fataftah A. Aflatoxin Decontamination of Artificially Contaminated Feeds by Sunlight, gamma Radiation and Microwave Heating. J. Applied Poultry Res. 2008; 17(4): 515-21.

Soliman KM. Incidence, level, and behavior of aflatoxins during coffee bean roasting and decaffeination. J. Agric. Food Chem. 2002; 50(25): 7477-81.

Arzandeh S. Jinap S. Effect of initial aflatoxin concentration, heating time and roasting temperature on aflatoxin reduction in contaminated peanuts and process optimisation using response surface modeling. Int J. Food Sci. Tech.. 2011; 46(3): 485-91.

Ogunsanwo BM, Faboya OOP, Idowu OR, Lawal OS, Bankole SA. Effect of roasting on the aflatoxin contents of Nigerian peanut seeds. Afric. J. Biotechnol. 2004; 3(9): 451-55.

Yazdanpanah H, Mohammadi T, Abouhossain G, Cheraghali M. Effect of roasting on degradation of Aflatoxins in contaminated pistachio nuts. Food Chem. Toxicol. 2005; 43(7): 1135-39.

Gowda NKS, Suganthi RU, Malathi V, Raghavendra A. Efficacy of heat treatment and sun drying of aflatoxin-contaminated feed for reducing the harmful biological effects in sheep. Anim. Feed Sci. Technol. 2007; 133(1-2): 167–75.

Park DL. Effect of processing on aflatoxin. Adv. Exp. Med. Biol. 2002; 504: 173-79.

Furtado RM, Pearson AM, Gray JI, Hogberg MG, Miller ER. Effect of cooking and/or processing upon levels of aflatoxins in meat from pigs fed a contaminated diet. J. Food Scie.. 2006; 46(5): 1306–08.

Midio AF, Campos RR, Sabino M. Occurrence of aflatoxins B1, B2, G1 and G2 in cooked food components of whole meals marketed in fast food outlets of the city of São Paulo, SP, Brazil. Food Addit. Contam. 2001; 18(5): 445–48.

Menon KRK. Zavier TV. Aflatoxin on ginger and ginger products and the effect of heating on their stability. As. J. Food Ag-Ind. 2010; 3(6): 562-66.

Shi SF, Chen W, Zhou Q, Li L, HaiTao Wu HT, Xing D, YuHua Bi YU. A study of the stability of AflatoxinB1 to several solutions through fluorescence spectral experiment, Biophotonics, Nanophotonics and Metamaterials, Metamaterials International Symposium on 16-18 Oct. 210 –13.

Mendez-Albores A, Del Rio-Garcia JC, Moreno-Martinez E. Decontamination of aflatoxin duckling feed with aqueous citric acid treatment. Anim. Feed Sci. Technol. 2007; 135(3-4): 249–62.

Mendez-Albores A, Martinez-Bustos F, Gaytan-Martinez M, Moreno-Martinez E. Effect of lactic and citric acid on the stability of B-aflatoxins in extrusion-cooked sorghum. Lett. Appl. Microbial. 2008; 47(1): 1-7.

Burgos-Hernandez A, Price RL, Jorgensen-Kornman K, López-García R, Njapau H, Park DL. Decontamination of aflatoxin B1-contaminated corn by ammonium persulphate during fermentation, J. Sci. Food Agric.. 2001; 82(5): 546–52.

Elias-Orozco R, Castellanos-Nava A, Gaytán-Martínez M, Figueroa-Cárdenas JD, Loarca-Piña G. Comparison of nixtamalization and extrusion processes for a reduction in aflatoxin content. Food Addit. Contam. 2002; 19(2): 878-85.

Prudente AD, King JM. Chemical detoxification of aflatoxins in food & feeds. Pp 543-554 in Abbas HK (ed) Aflatoxin and Food Safety. New York: CRC Press, 2005

Nyandieka HS, Maina JO, Nyamwange C. Destruction of Aflatoxins in Contaminated Maize Samples using Ammoniation Procedures. East Central African J. Pharm. Sci. 2009; 12(3): 47-51.

Williams JH, Phillips TD, Jolly PE, Stiles JK, Jolly CM, Aggarwal D. Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. Am. J. Clin. Nutr. 2004; 80(5): 1106-22.

Karaca H, Nas S. Combined effect of pH and heat treatment on degradation of aflatoxins in dried figs. J. Food Process Preserv. 2009; 33(suppl. 1): 329‒39.

Jalili M, Jinap S. Role of sodium hydrosulphite and pressure on the reduction of aflatoxins and ochratoxin A in black pepper, Food Cont. 2012; 27(1), 11-15.

Jalili M, Jinap S. Reduction of mycotoxins in white pepper. Food Addit. Contam: Part A. 2012; 29(12) 1–12.

Freitas-Silva O, Venancio A. Ozone applications to prevent and degrade mycotoxins: a review. Drug Metab. Rev. 2010; 42(4): 612-20.

El-Desouky TA, Sharoba AMA, El-Desouky AI, El-Mansy HA, Naguib K. Effect of ozone gas on degradation of aflatoxin B1 and aspergillus flavus fungal. J. Environ. Anal. Toxicol. 2012; 2(1): 128.

Luo X, Wang R, Wang L, Li Y, Bian Y, Chen Z. Effect of ozone treatment on aflatoxin B1 and safety evaluation of ozonized corn. Food Cont. 2014; 37(1): 171-76.

De Alencar ER, Faroni LR, Soares Nde F, da Silva WA,Carvalho MC. Efficacy of ozone as a fungicidal and detoxifying agent of aflatoxins in peanuts. J. Sci. Food Agric., 2012; 92(4): 899-05.

Fazeli MR, Hajimohammadali M, Moshkani A, Samadi N, Jamalifar H, Khoshayand MR. Aflatoxin B1 binding capacity of autochthonous strains of lactic acid bacteria. J. Food Prot. 2009; 72(1):189–92.

Yin Y, Yan L, Jiang J, Ma Z. Biological control of aflatoxin contamination of crops. J. Zhejiang Univ Sci B. 2008; 9(10): 787–92.

Fuchs S, Sontag G, Stidl R, Ehrlich V, Kundi M, Knasmüller S. Detoxification of patulin and ochratoxin A, two abundant mycotoxins, by lactic acid bacteria. Food Chem. Toxicol. 2008; 46(4):1398-07.

Oluwafemi F, Da-Silva FA. Removal of aflatoxins by viable and heat-killed lactobacillus species isolated from fermented maize. J. Appl. Biosci. 2009; 16(1): 871-76.

Kim JG. Anti-aflatoxigenic activity of some bacteria related with fermentation. Communicating Current Research and Educational Topics and Trends in Applied Microbiology, 2007;1: 322-28.

Nesci AV, Bluma RV, Etcheverry MG. In vitro selection of maize rhizobacteria to study potential biological control of Aspergillus section Flavi and aflatoxin production. Eur. J. Plant. Pathol. 2005; 113(2): 159–71.

Jermnak U, Chinaphuti A, Poapolathep A, Kawai R, Nagasawa1 H, Sakuda S. Prevention of aflatoxin contamination by a soil bacterium of Stenotrophomonas sp. that produces aflatoxin production inhibitors. Microbiol. 2013; 159(5): 902–912.

Palumbo JD, Baker JL, Mahoney NE. Isolation of bacterial antagonists of Aspergillus flavus from almonds. Microb. Ecol. 2006; 52(1): 45–52.

Dorner JW. Biological control of aflatoxin contamination of crops. J. Toxicol. Toxin. Rev. 2004; 23(2-3): 425–50.

Masoud W, Kaltoft CH. The effect of yeasts involved in the fermentation of coffee arabica in East Africa on growth and ochratoxin A (OTA) production by aspergillus ochraceus. Int. J. Food Microbiol. 2006; 106(2): 229-34.

Bueno D, Casale CH, Pizzolitto RP, Salano MA, Olivier G. Physical adsorption of aflatoxin B1 by lactic acid bacteria and Saccharomyces cerevisiae: a theoretical model. J. Food Prot. 2007; 70(9): 2148-54.

Guan S, Gong M, Yin Y, Huang R, Ruan Z, Zhou T, Xie M. Occurrence of mycotoxins in feeds and feed ingredients in China. J. Food Agric. Environ. 2011; 9(2): 163-67

Prado G, Cruz Madeira JEG, Morais VAD. Reduction of aflatoxin B1 in stored peanuts (Arachis hypogaea L.) using Saccharomyces cerevisiae. J. Food Prot. 2011; 74(6): 1003–06.

Milani J, Seyed Nazari SS, Bamyar E, Maleki G. Effect of Bread Making Process on Aflatoxin Level Changes. J. Chem. Health Risks. 2014; 4(4): 1-7

Motawe HFA, Abdel Salam AF. Reduction the toxicity of aflatoxin in broiler chickens’ diet by using probiotic and yeast. Int. J. Poultry Scie.. 2014; 13(7): 397-07.

Rajani P, Sridevi V, Chandana Lakshmi MVC. A review on biological control of aflatoxin crop contamination. IJCEPr. 2012; 3(1): 83-86.

Dorner JW, Cole RJ, Connick WJ, Daigle DJ, Michael R, McGuire C, Baruch S. Evaluation of biological control formulations to reduce aflatoxin contamination in peanuts. Biol .Control. 2003; 26(3): 318–24.

Atehnkeng J, Ojiambo PS, Donner M, Ikotun T, Sikora RA, Cotty PJ, Bandyopadhyay R Distribution and toxigenicity of Aspergillus species isolated from maize kernels from three agro-ecological zones in Nigeria. Int. J. Food Microbiol. 2008; 122(1-2): 74-84.

Pitt JI, Hocking AD. Mycotoxins in Australian: biocontrol of aflatoxin in peanuts. Mycopathologia. 2006; 162(3): 233-243.

Haskard CA, El-Nezami HS, Kankaanpaa PE, Salminen S, Ahokas JT. Surface binding of aflatoxin B1 by lactic acid bacteria. Appl. Environ. Microbiol. 2001; 67(7): 3086-91.

EI-Nezarni H, Mykkiinen H, Kankaanpi H, Salminen S, Ahokas J. Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum. J. Food Prot. 2000; 63(4): 549-52.

Gratz S, Mykkanen H, EI-Nezarni H. Aflatoxin B1 binding by a mixture of Lactobacillus and Propionibacterium: in vitro versus ex vivo. J. Food Prot. 2005; 68(11): 2470-74.

Peltonen K, EI-Nezami H, Haskard C, Ahokas J, Salminen S. Aflatoxin B1 binding by dairy strains of lactic acid bacteria and bifidobacteria. J. Dairy Sci. 2001; 84(10): 2152-56.

Topcu A, Bulat T, Wishah R, Boyaci IH. Detoxification of aflatoxin B1 and patulin by Enterococcus faeclum strains. Int. J. Food Microbiol. 2010; 139(3): 202-05.

Shetty PH, Jespersen L. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends in Food Sci. Technol. 2006; 17(2):48-55.

Raju MVLN, Devegowda G. Esterified-glucomannan in broiler chicken diets-contaminated with aflatoxin, ochratoxin and T-2 toxin: evaluation of its binding ability (in vitro) and efficacy as immunomodulator. Asian-Aust. J. Animal Sci. 2002; 15(7): 1051-56.

Shetty PH, Hald B, Jespersen L. Surface binding of aflatoxin B 1 by Saccharomyces cerevisiae strains with potential decontaminating abilities in indigenous fermented foods. Int. J Food Microbiol. 2007; 113(1): 41-46.

Abbas HK, Zablotowicz RM, Horn BW, Phillips NA, Johnson BJ, Jin X. Comparison of major biocontrol strains of non-aflatoxigenic Aspergillus flavus for the reduction of aflatoxins and cyclopiazonic acid in maize. Food Addit. Contam. Part A. 2012; Chem. Anal. Control Expo., 2011;8(2): 198-08

Dorner JW. Biological control of aflatoxin contamination in corn using a nontoxigenic strain of Aspergillus flavus. J Food Prot. 2009; 72(4): 801–04.

Dorner JW. Efficacy of a biopesticide for control of aflatoxins in Corn. J. Food Prot. 2010; 73(3): 495–99

Alaniz Zanon MS, Chiotta ML, Giaj-Merlera G, Barros G, Chulze S. Evaluation of Potential Biocontrol Agent for aAflatoxin in Argentinean Peanuts, Int. J. Food Microbiol., 2013; 162(3): 220-25.

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